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In vitro shoot tip culture of N. campanulata

Very grateful Hardy, thank you for sharing your methods and results. Years ago I tried soaking Cephalotus leaves in a milky captan solution, and then bleaching them, but it never worked for me. (However my bleach solution was much stronger than yours.) I'll have to try this with Nepenthes--
 
Just an update, here's two of the best-looking cultures.

35848908080_4a7f67ed46_z.jpg


These were subcultures starting with similar-sized single shoots, using multiplication medium. One has produced fewer but larger shoots, the other has proliferated more into a mass of tiny shoots.

36107248311_3f96933c2c_z.jpg
36241715585_ac47b0fc7e_z.jpg


I haven't tried dividing and subculturing single shoots onto hormone-free medium. Based on the experience of others this would cause the divided shoot to grow normally and begin pitchering instead of proliferating. I will try it soon.




Last update as of 2017/7/30: unfortunately all cultures are dead due to neglect.
 
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