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DerbyCityNeps
Always a newbie- Previously glider14
im guessing your talking about tissue culture? ive never done tissue culture nor do i know much about it. but unflasking as far as i know is taking the plant from its sterile controled environment and putting it into somthing of high humidity(like a greenhouse) this process will become greenhouse hardening off. the plants are then shipped in fresh growing media to stores around the world.
i hope that helps
Alex
i hope that helps
Alex
BigCarnivourKid
It's been one of dem days
Hi Alec,
Plants propagated by Tissue Culture, are grown in flasks. The flask contains sterile media in which the plant(s) grow(s) until ready to be "hardened off". To harden off a deflasked plant, you rinse the sterile media off the plant and place it in the proper soil and keep the humidity high. The plants will also be sensitive to direct sunlight, so they need to be gradually hardened to that also. Over a period of time the humidity is lowered and the amount of sunlight is increased until the plants can handle whatever mother nature throws at them.
Not all plants will survive the process.
Plants propagated by Tissue Culture, are grown in flasks. The flask contains sterile media in which the plant(s) grow(s) until ready to be "hardened off". To harden off a deflasked plant, you rinse the sterile media off the plant and place it in the proper soil and keep the humidity high. The plants will also be sensitive to direct sunlight, so they need to be gradually hardened to that also. Over a period of time the humidity is lowered and the amount of sunlight is increased until the plants can handle whatever mother nature throws at them.
Not all plants will survive the process.