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Heli pollen observations

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BigBella said:
I feel somehow dirty after that pollen tube spectacle -- but compelled to look as always. Three stars; I'd give it four but don't wish to further encourage Butch's smut-peddling . . .
http://www.youtube.com/watch?v=6DVmfd1x5L8&feature=related
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you silly man, I didnt post the NC17 version.... that cost $4.99USD to view

snapperhead51 said:
very nicly done Butch , never seen heli pollen before up close and personal , thanks for the effort mate very cool indeed
J
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Thanks John, fun project.... and uber easy once I had the right resources and technique.

I hope to determine the "shelf life" of heli pollen and the effectiveness of common storage techniques

:D
 
Not a Number said:
I trust an article in CPN is in the works.
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This is a 'must-do'! Looking forward to seeing you published. Bob Z. can facilitate your efforts ....

Congrats on the success. :hail:

Holler if you think you might want to try some U. asplundii pollen.
 
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Av8tor1 said:
you silly man, I didnt post the NC17 version.... that cost $4.99USD to view



Thanks John, fun project.... and uber easy once I had the right resources and technique.

I hope to determine the "shelf life" of heli pollen and the effectiveness of common storage techniques

:D
Click to expand...

Ok mate that would be very cool indeed , look forward to your pending as time allows results
let me be on your number one let know list please
thanks
J
 
Confirmation, Heli pollen can be stored frozen and remain viable!

Sample dried for two days in fridge using desiccant then froze for 7 days... thawed and checked for tube growth.

Growth observed :)

Now the next question.... but for how long?

woot woot!
 
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"Woot, woot," indeed. Congratulations. I'll bring a marine air-horn to the ICPS conference . . .

Next, cold fusion . . .
 
Scales arrived today....

Now waiting on research grade boric acid and calcium nitrate.

Heli pollen tube research part deux; I need to come up with a media of unquestionable repeatability with better imaging characteristics.
I not only want to check for tube growth, but also hope to quantify percentages.

:p
 
100g/L Sucrose (10%)
100mg/L Boric Acid
300 mg/L Calcium Nitrate

No growth...
(Interesting, I was expecting to see growth with this media....)

2-5% will be next attempt

Onion epidermis may be the way I have to go
:p
 
Av8tor1 said:
100g/L Sucrose (10%)
100mg/L Boric Acid
300 mg/L Calcium Nitrate

No growth...
(Interesting, I was expecting to see growth with this media....)

2-5% will be next attempt

Onion epidermis may be the way I have to go
:p
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Perhaps attempting to duplicate the amount of sucrose common to TC media would be of help. 100 g/L of sucrose is well over three times the maximum amount for any media . . .
 
most refs mention 20%.... so that is where i started, but working my way down from there

ill try 2% Thursday :)

ummmm so what is the TC sucrose level?

but i doubt an onion epidermis is anywhere near 10-20% (at least that is what hillbilly common sense tells me)
 
Av8tor1 said:
most refs mention 20%.... so that is where i started, but working my way down from there

ill try 2% Thursday :)

ummmm so what is the TC sucrose level?

but i doubt an onion epidermis is anywhere near 10-20% (at least that is what hillbilly common sense tells me)
Click to expand...

The typical amount of sucrose is 25-30 g/L . . .
 
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