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Tissue Cultured VFT's

jimscott

Tropical Fish Enthusiast
Last weekend I received a jar of TC VFT's, with a link to how to transition them to the real world. It was quite elaborate, with the tools (forceps, scissors,..) Basically, I poured the contents in a spaghetti collander and ran warm water through the plants. I could see numerous small chunks of what I believe to be the culture medium, amid the plant clumps and scattered VFT leaves. I was able to pick and separate plant from medium.

Picture044.jpg


Picture086-2.jpg
 
VFT rooting cultures are usually just Jello and part of a multi vitamin pill. Eat away.

Here was my culture.

<table style="width:auto;"><tr><td><a href="http://picasaweb.google.com/lh/photo/2K6r3Nob6A57TtIwm0fvqg?authkey=Gv1sRgCOvG6tXP0_HD4wE&feat=embedwebsite"><img src="http://lh6.ggpht.com/_PMY_wtnw6jQ/SaGaNmqKTZI/AAAAAAAAAoA/jVf1QYbXprE/s400/P2170024.JPG" /></a></td></tr><tr><td style="font-family:arial,sans-serif; font-size:11px; text-align:right">From <a href="http://picasaweb.google.com/nateniu/TissueCultureClass?authkey=Gv1sRgCOvG6tXP0_HD4wE&feat=embedwebsite">Tissue Culture Class</a></td></tr></table>
 
VFT rooting cultures are usually just Jello and part of a multi vitamin pill. Eat away.

Here was my culture.

<table style="width:auto;"><tr><td><a href="http://picasaweb.google.com/lh/photo/2K6r3Nob6A57TtIwm0fvqg?authkey=Gv1sRgCOvG6tXP0_HD4wE&feat=embedwebsite"><img src="http://lh6.ggpht.com/_PMY_wtnw6jQ/SaGaNmqKTZI/AAAAAAAAAoA/jVf1QYbXprE/s400/P2170024.JPG" /></a></td></tr><tr><td style="font-family:arial,sans-serif; font-size:11px; text-align:right">From <a href="http://picasaweb.google.com/nateniu/TissueCultureClass?authkey=Gv1sRgCOvG6tXP0_HD4wE&feat=embedwebsite">Tissue Culture Class</a></td></tr></table>

Hey, there's always room for Jello . . .
 
Are those typical VFTs in the TC vessel that you got? Where did you get them?

Good luck on the deflasking process. I haven't had to do this yet. So far I still have all of my TC work in jars. It might be ready to come out in a few months though.
 
I have found that TC VFTs received in agar are pretty hardy. I gently wash the mess in water and separate those that come apart easily. I then plant those that are still firmly clumped as a clump. In a few months, individual plants can be more easily separated from the clump and repotted. In this way, very few plantlets die.

Anyway, that is what I do.
 
I have found that TC VFTs received in agar are pretty hardy. I gently wash the mess in water and separate those that come apart easily. I then plant those that are still firmly clumped as a clump. In a few months, individual plants can be more easily separated from the clump and repotted. In this way, very few plantlets die.

Anyway, that is what I do.

And that is what I did.
 
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