Tissue Culture Reference?

[Vbkid]

So I recently purchased some N. Truncata seed that I will be receiving in a week or so. Expecting about 50 seed, I thought his might be a good opportunity to make an attempt at a sterile tissue culture medium for some of them. While I've seen and read a bit on TC, I was wondering if anyone could recommend some good references and/or places for supplied.

Thanks,
Kyle

 

[dionae]

I ordered some of the same seed for the same reason. Heres a few links that might help. Good luck!

http://www.bio.davidson.edu/people/kabernd/seminar/2002/method/amy/aj.htm

http://www.scribd.com/doc/3544465/Plant-Tissue-Culture

http://tech.groups.yahoo.com/group/hometissueculture/

http://www.triffidpark.com.au/htm_pages/tissue_culture_information.htm

http://www.omnisterra.com/botany/cp/slides/tc/tc.htm

http://www.molecular-plant-biotechnology.info/plant-tissue-culture/culture-medium-for-meristem-culture.htm

Sorry if none of these have seed protocols but you should at least have a basic understanding of it.

http://www.archive.org/stream/handbookofplantt00whit/handbookofplantt00whit_djvu.txt

http://www.ccebook.org/preview/0881923613/Plants-from-Test-Tubes-An-Introduction-to-Micropropagation

http://csm.jmu.edu/biology/renfromh/pop/pctc/tcstart.htm

 

[Vbkid]

Anyone have any advice on what media is best to use for Nepenthes TC?

 

[dionae]

I've read 1/3 MS...same with VFTs but i'm just an amateur so...

I'm going to try 1/3 MS with mine.

 

[dionae]

I just started trying TCing in January so I have limited experience and minimal success. Heres some VFTs that I germinated in vitro using 1/3 MS. I only wish I could get a strike on one of my explants.

 

[dionae]

Heres a nice thread on TCing neps.

http://icps.proboards.com/index.cgi?board=tissue&action=display&thread=3147