Hello Everyone!
I'm not even sure if "Agar Hydroponics" is the correct word, but let me outline for you what I'm planning. For Christmas, I got a "Bacteria Growing Kit" with the petri dishes and agar powder. And as another hobby, I maintain cultures of the bioluminescent dinoflagellate Pyrocystis fusiformis I have vials of concentrated Sodium Phosphate, Sodium Nitrate, F/2 Trace metals and vitamins, and Sodium Silicate. For my dinos, I put about one mL of these per liter of medium (except the vitamins, which is .5mL/L).
My plan was to use the agar, with the addition of the nutrients (barring the silicate, which was for when I had diatoms) to grow, say, D. capensis from seed in vitro, after washing them with the classic 10% hydrogen peroxide solution. Do you think this would be crazy enough to work? Or am I missing something? I have grown Arabadopsis thaliana seeds in vitro before, but our instructor prepared both the media and the seeds, and I want to give this a shot, being the eager young scientist that I am...
Tell me what you think,
CJ
I'm not even sure if "Agar Hydroponics" is the correct word, but let me outline for you what I'm planning. For Christmas, I got a "Bacteria Growing Kit" with the petri dishes and agar powder. And as another hobby, I maintain cultures of the bioluminescent dinoflagellate Pyrocystis fusiformis I have vials of concentrated Sodium Phosphate, Sodium Nitrate, F/2 Trace metals and vitamins, and Sodium Silicate. For my dinos, I put about one mL of these per liter of medium (except the vitamins, which is .5mL/L).
My plan was to use the agar, with the addition of the nutrients (barring the silicate, which was for when I had diatoms) to grow, say, D. capensis from seed in vitro, after washing them with the classic 10% hydrogen peroxide solution. Do you think this would be crazy enough to work? Or am I missing something? I have grown Arabadopsis thaliana seeds in vitro before, but our instructor prepared both the media and the seeds, and I want to give this a shot, being the eager young scientist that I am...
Tell me what you think,
CJ