Cephalotus in TC Media . . .

[BigBella]

Every other year or so, I divide up my Cephalotus plants and take a few leaf and rhizome cuttings; and here is a tiny piece suspended in 1/2 strength MS media, now exhibiting new growth. Apologies for the blurred second photo; or as my father once said, "It's a poor worker who blames his tools . . ."

Cephalotus follicularis cv. "Hummer's Giant"

 

[jht-union]

Nice!

 

[cpsammich]

cool

 

[mass]

Congrats David. Well done..

 

[Sig]

Very cool! Any hormones?

 

[Api]

How long ago did you put the piece in TC?

 

[BigBella]

Very cool! Any hormones?

Yes, 0.1 mg/L of NAA . . .

How long ago did you put the piece in TC?

The elapsed time is about three weeks . . .

 

[Api]

That's pretty quick.

 

[lance]

Cool!
How exactly do you do that and with what solutions?
So far I know the enviroment has to be sterile and have 3% succrose.
I am very interested in doing this for trading plants

 

[Sig]

Cool!
How exactly do you do that and with what solutions?
So far I know the enviroment has to be sterile and have 3% succrose.
I am very interested in doing this for trading plants

http://www.flytrapcare.com/tissue-culture-basics.html

Very, very sterile... probably the hardest part of the process. FTC explains everything.

 

[BigBella]

Cool!
How exactly do you do that and with what solutions?
So far I know the enviroment has to be sterile and have 3% succrose.
I am very interested in doing this for trading plants

It is far too involved for a detailed explanation here. I would instead refer you to www.flytrapcare.com and its tissue culture forum. There is plenty of information to be had at that site -- everything from safety procedures; the maintaining of sterility; to the mixing of media . . .