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D. regia

Joined
Oct 28, 2002
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Ontario
The following pictures are not very clear as I had to take them through the tissue culture bottles. In any case you should be able to make out in some of them the D.regia seeds just starting to sprout.


tcregia0001.jpg
tcregia0002.jpg

tcregia0003.jpg

tcregia0004.jpg
 
Joined
Sep 21, 2002
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Baltimore z7b
Yeah, Tissue culture seems like something that would be fun to experiment. With does anyone know how much they cost? Or a website that teaches lists what you need to make a kit. It's all so....neat! Then maybe I can actually germinate some byblis and neps seeds! That would be great!
 
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Can I ask how you sterilize the air in it and put the seeds+jell in it fast enough without contaminating it? I wish you success with the seeds
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. Zongyi
 

Tom

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Jan 30, 2003
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Quebec city, Qc, Canada
Zongyi_Yang: If you do your manipulation in a 'almost closed' container (such as a fish tank, on its side), and work close enough from a flame (ie alcohol lamp), you can achieve to reduce/avoid contamination to almost nothing. Of course, you must have a very clean area (ie the container) and washed your hands and arms properly before doing anything. Plus avoiding air circulation in your room during the transfert.
 
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Zongyi_Yang

To answer some of your questions:

The jelly and air in the bottle are sterilized in a large pressure cooker. The work area ( table top, clean box and even the air in the room) are disinfected with a number of house hold chemicals such as rubbing alcohol & Lysol. Lastly the seeds are sterilized in diluted bleach and or hydrogen peroxide.

So far I have had very low rate of contamination, and the one that did get contaminated was a ceph root ( which I had guessed from the begging would not be sterile).

Andre
 
Joined
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Hehe, sounds tricky. Maybe I will get one someday too, but currently, I will rather spend the money on other stuff or more cps
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. I mean, it's not really the cheapest
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. Zongyi
 
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