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Byblis liniflora germination....
- Thread starter ChronoKiento
- Start date
jimscott
Tropical Fish Enthusiast
Byblis liniflora seed can be germinated in just a few days (3-4) if you first soak the seed in hot water and then use a weak bleach solution to remove all the dark purple pigment from the seed just prior to sowing them. Apparently the pigment in the seed testa (seed coat) proves to be a germination inhibitor, though a weak one. After the pigment is rinsed out don't forget a final rinse with plain water to remove the remaining bleach solution.
1::10; 1 part bleach to 10 parts water
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I first put the seeds in a tea cup, pour water (hot, but not too hot to touch) enough to cover the seed about 1 inch deep. Let them soak for several hours (this is the first stage of germination -- called imbibition) to initiate the germination process. Then I put a paper towel in a plastic funnel, pour the seed out onto the paper in the funnel so the liquid drains off, then I used a plastic irrigation bottle with a 1::10 bleach solution to wash the seed until no more purple pigment leached from the seed. I then returned the seed to the cup and soaked them again in water to rinse off most of the bleach. Then I sowed them on the surface of moist peat moss and placed them into a ziploc bag under fluorescent lights. They germinated in less than 24 hours.
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I discovered this by accident, when I was preparing some seed to sow in vitro I noticed there was purple leaching into the paper towel from the seed, so I continued the bleach rinse until it had all leached out of the seed and into the paper towel. After preparing the seed to sow I then planted half in moist peat moss and the other half in petri dishes of sterile nutrient agar. Wow, was I surprised when they all germinated within 24 hours.
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I first put the seeds in a tea cup, pour water (hot, but not too hot to touch) enough to cover the seed about 1 inch deep. Let them soak for several hours (this is the first stage of germination -- called imbibition) to initiate the germination process. Then I put a paper towel in a plastic funnel, pour the seed out onto the paper in the funnel so the liquid drains off, then I used a plastic irrigation bottle with a 1::10 bleach solution to wash the seed until no more purple pigment leached from the seed. I then returned the seed to the cup and soaked them again in water to rinse off most of the bleach. Then I sowed them on the surface of moist peat moss and placed them into a ziploc bag under fluorescent lights. They germinated in less than 24 hours.
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I discovered this by accident, when I was preparing some seed to sow in vitro I noticed there was purple leaching into the paper towel from the seed, so I continued the bleach rinse until it had all leached out of the seed and into the paper towel. After preparing the seed to sow I then planted half in moist peat moss and the other half in petri dishes of sterile nutrient agar. Wow, was I surprised when they all germinated within 24 hours.
PinguiculaMan, that's amazing!!! I'll have to try that when my byblis gives me some seeds! It's a fairly simple process too! I have one question though, what's an irrigation bottle?
You should really get these instructions put on the drosera forum as a sticky, I know a lot of people don't know how to germinate byblis correctly and this is a huge breakthrough! Germination in 24 hours! WOW!
You should really get these instructions put on the drosera forum as a sticky, I know a lot of people don't know how to germinate byblis correctly and this is a huge breakthrough! Germination in 24 hours! WOW!
[b said:
Here is one style: http://www.southwestmedical.com/ab15880....ATID=61
and others:
http://www.labdepotinc.com/Category_Details~id~370.aspx
Perhaps I should have asked the following question earlier (a bit of backgroud info first):
I sowed B.liniflora seed on 7-1-05. The seed were placed in GA3 for 24h and thereafter placed on the surface of a 1:1 peat:sand mix covered by translucent plastic. The pots are kept constantly moist (sitting in an open water tray) 5" under 80W cool white fluorescent lights (15h photoperiod).
It's been ~1 month, and none of the seed have germinated. The seed appear normal and I've been told patience is a necessary virtue with byblis seed!
However, I was wondering whether your (PinguiculaMan) method could be applied to the seed now? Have you ever tried this method on already-sown seed?
Thanks,
I sowed B.liniflora seed on 7-1-05. The seed were placed in GA3 for 24h and thereafter placed on the surface of a 1:1 peat:sand mix covered by translucent plastic. The pots are kept constantly moist (sitting in an open water tray) 5" under 80W cool white fluorescent lights (15h photoperiod).
It's been ~1 month, and none of the seed have germinated. The seed appear normal and I've been told patience is a necessary virtue with byblis seed!
However, I was wondering whether your (PinguiculaMan) method could be applied to the seed now? Have you ever tried this method on already-sown seed?
Thanks,
