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Recent content by Anne-Lise

  1. Anne-Lise

    Going all-out to germinate some Drosophyllum

    Okay, so I'm jumping into the conversation a little late but for those like me who are looking for info about Drosophyllum and the calcium debate, I thought this fairly recent paper might help. It's a great paper that gives details about soil, germination technique and the effect of...
  2. Anne-Lise

    Ongoing project: sequencing the genome of an Alaskan Utricularia

    Thank you patrickntd. I hope that you're doing okay in Houston. My thoughts are going to you and your dears.
  3. Anne-Lise

    Ongoing project: sequencing the genome of an Alaskan Utricularia

    Excellent point ! I found this paper a couple of days ago and I am definitely keeping my eyes open on Tetrahymena and its algae: https://www.ncbi.nlm.nih.gov/pubmed/27613221 A month ago when I wasn't aware of this paper, I blasted some reads reads and I remember seeing some ciliophora hits which...
  4. Anne-Lise

    Ongoing project: sequencing the genome of an Alaskan Utricularia

    I assembled the genome with Canu and Minimap/miniasm to compare the results of the 2 assemblers. They might be OLCs. They're pretty standard for Nanopore reads. Assemblies went fast and pretty well I guess. I've got no Illumina reads so I polished with the Nanopore reads and Racon (another...
  5. Anne-Lise

    Ongoing project: sequencing the genome of an Alaskan Utricularia

    emc2: Yeah, I watched the talk they gave at the London Calling in 2016. It got me really excited. I was not aware that they did a Nature paper with it. It's awesome ! Thank you !!! I didn't read the paper yet but I think they used a older generation chemistry which means that we get even better...
  6. Anne-Lise

    Ongoing project: sequencing the genome of an Alaskan Utricularia

    Well, thank you Tanukimo, it helps indeed. This phylogeny is pretty recent. Thus, it is not necessary for me to reconstruct one except for the case I came to radically different results.
  7. Anne-Lise

    Ongoing project: sequencing the genome of an Alaskan Utricularia

    Hello all, As I already introduced it in the "ID this plant" section, I recently sequenced the entire genome of an Utricularia that grows around here (Fairbanks, AK). From the look of the pictures I posted in the ID thread, it seems that we're dealing with U. macrorhiza but this will be...
  8. Anne-Lise

    Utricularia from wetlands in Alaska

    Hello guys, To reconstruct phylogenies, you need to compare between species something they have in common (gene(s) or protein(s)). I saw >50 sequences of diverse utricularias of the NCBI database and I will extract from my own genome the gene/protein that has been the most deposited in the...
  9. Anne-Lise

    Utricularia from wetlands in Alaska

    jeff 2: I will have no spur picture for this year. The blooming is over. I think we will get confirmation with the DNA pretty soon now. :-D
  10. Anne-Lise

    Utricularia from wetlands in Alaska

    Thank you Tanukimo for this additional criterium related to the absence of roots. jeff 2: Here is a microscopy of a bladder. We can see a bunch of quadrifidal hairs floatting around. Another strong indication of U. macrorhiza it seems. Yesterday, I participated to a fish survey in the Chena...
  11. Anne-Lise

    Utricularia from wetlands in Alaska

    I used http://www.zupimages.net/ instead of Google drive.
  12. Anne-Lise

    Hello everybody !

    Indeed ;-)
  13. Anne-Lise

    Utricularia from wetlands in Alaska

    Hello Dexenthes and you all, Thank you for following up on this ID. The UAF herbarium (ARCTOS: http://arctos.database.museum/SpecimenSearch.cfm) reports Utricularia all over AK ! Once, I get mine IDed, I would love to know if it is the same species than your, Dexenthes ! Jeff 2 wanted to see...
  14. Anne-Lise

    Utricularia from wetlands in Alaska

    nimbulan: Yes, indeed the pics were on a Google drive. Sorry for that. I edited the post. Tanukimo: that is a very efficient criterion of identification for macrorhiza. With the pictures now available, we can see that there is bladders and foliage on the same stem. However, I've got more...
  15. Anne-Lise

    Utricularia from wetlands in Alaska

    Hi Jeff, I saw somewhere that there's a couple of pinguicula growing in Alaska and I would like to find them before winter (that is kind of like in 6 weeks - LOL). The minION is user-friendly compared to the other sequencing technologies but it still requires lab skills in molecular biology...
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